Identification of leptospira serovars by restriction-endonuclease analysis
نویسندگان
چکیده
منابع مشابه
Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA.
Primers for PCR were selected from a sequenced fragment of clone pL590, which contains a repetitive element present in the genome of Leptospira interrogans serovar hardjo type hardjoprajitno (M. L. Pacciarini, M. L. Savio, S. Tagliabue, and C. Rossi, J. Clin. Microbiol. 30:1243-1249, 1992). A specific DNA fragment was amplified from the genomic DNAs of serovar hardjo type hardjoprajitno and nin...
متن کاملRestriction endonuclease DNA analysis of Leptospira interrogans serovars icterohaemorrhagiae and hebdomadis.
Antigenic variants of Leptospira interrogans serovars copenhageni and hebdomadis were examined by bacterial restriction endonuclease DNA analysis with EcoRI, XhoI, SalI, BstEII, and HindIII as the digesting enzymes. The antigenic variants were stable cloned strains which had been cultivated in media containing homologous immune serum. One of the strains examined has been reported elsewhere (R. ...
متن کاملIsolation and identification of restriction endonuclease BsiSI.
BseBI, an isoschizomer of BstNI (1) has been purified from Bacillus stearothermopilus species. BseBI recognises the sequence 5'...CCWGG...3' (W=A or T) and cleaves between C and W. The enzyme was purified using the following Chromatographic steps: 1. Blue -Sepharose F3GA, 2. Heparin -Sepharose, 3. DEAE-Sepharose. The enzyme was free of contaminating nuclease activity. After 5-fold overdigestion...
متن کاملIsolation and identification of restriction endonuclease SseAI.
SseBI, an isoschizomer of StuI (1) has been purified from Streptomyces species. SseBI recognises the sequence 5' ... AGGCCT ... 3' and cleaves between G and C. The enzyme was purified using the following chromatographic steps: 1. Blue Sepharose F3GA, 2. Heparin-Sepharose. The enzyme was free of contaminating nuclease activity. After 100 fold overdigestion on lambda DNA greater than 95% of the D...
متن کاملIdentification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Leptospires are usually classified by methods based on DNA-DNA hybridization and the conventional cross-agglutination absorption test, which uses polyclonal antibodies against lipopolysaccharides. In this study, the amplification of the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify Leptospira. DNA extracts from sixty-eight serovars were...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 1981
ISSN: 0022-2615,1473-5644
DOI: 10.1099/00222615-14-1-163